TEDE Coleção: Mestrado em Ciência AnimalMestrado em Ciência Animalhttp://bdtd.unoeste.br:8080/jspui/handle/tede/6212024-03-28T19:23:00Z2024-03-28T19:23:00ZImpactos da venlafaxina na espermatogênese e fertilidade masculina: revisão sistemática em modelo experimentalSilva, Carolina Galantehttp://bdtd.unoeste.br:8080/jspui/handle/jspui/15602024-03-16T04:01:42Z2023-08-08T00:00:00ZTítulo: Impactos da venlafaxina na espermatogênese e fertilidade masculina: revisão sistemática em modelo experimental
Autor: Silva, Carolina Galante
Primeiro orientador: Castilho, Anthony César de Souza
Abstract: Antidepressant medications are commonly used in men of reproductive age for the long-term treatment of depression, as well as other disorders. Although antidepressants are associated with negative sexual effects, such as reduced libido, sexual impotence, anorgasmia or ejaculatory delay, the literature lacks clear evidence on the role of this therapy on male fertility. Venlafaxine is a serotonin and noradrenaline reuptake inhibitor antidepressant with high efficacy and tolerability, being widely used in clinical practice. Thus, our aim was to review and analyze the impact of venlafaxine on aspects of male fertility using experimental male murine models through a systematic review. To do so, we conducted a survey in three literature databases. Initially, we found 84 articles in searches by title and abstract on PubMed (n = 6), Embase (n = 47) and Scopus (n = 31) platforms. As inclusion criteria, we considered controlled and randomized studies in male mice, submitted to the use of venlafaxine, covering all languages and an open period. In the exclusion criteria, we considered the presence of comorbid diseases and studies in females. After removal of duplicates and thorough and complete analysis, 9 articles were included in this review. The implied results showed a negative impact on sperm concentration, hormonal dysregulation, changes in the histoarchitecture of the reproductive system, and a large increase in oxidative stress. In conclusion, we observed that venlafaxine negatively affects the fertility of male mice.
Instituição: Universidade do Oeste Paulista
Tipo do documento: Dissertação2023-08-08T00:00:00ZExpressão de genes antioxidantes em testículos de ratos submetidos ao envenenamento experimental agudo por Lachesis muta muta (Surucucu Sul-Americana) em ratos tratados com n-acetilcisteína e antivenenoTeixeira, Regina Rafaelhttp://bdtd.unoeste.br:8080/jspui/handle/jspui/15592024-03-16T04:01:47Z2023-09-22T00:00:00ZTítulo: Expressão de genes antioxidantes em testículos de ratos submetidos ao envenenamento experimental agudo por Lachesis muta muta (Surucucu Sul-Americana) em ratos tratados com n-acetilcisteína e antiveneno
Autor: Teixeira, Regina Rafael
Primeiro orientador: Giometti, Ines Cristina
Abstract: There is evidence in the literature that the snakebite can cause changes in spermatogenesis, increased testicular oxidative stress, testicular atrophy and endocrine changes. Despite this, there are no studies about reproductive consequences for men on envenomation by Lachesis. The treatment of envenomation is conditioned to serum therapy and there are few studies about the use of other therapeutic strategies. N-acetylcysteine (NAC) is an antioxidant that could help reduce testicular oxidative stress, but it has not yet been tested for this purpose. The aim of this study was to evaluate the use of NAC, associated or not with anti-Bothrops/Lachesis serum, on the expression of antioxidant genes in the testicles of rats submitted to experimental envenomation by Lachesis muta muta (Surucucu Sul-Americana). Wistar rats were divided into 5 groups (n=6): V = venom; V + S = venom and serum; NAC = only NAC without venom; V+NAC = venom and NAC (V+NAC); and V+S+NAC = venom, NAC and antivenom serum. The venom was applied in a single dose of 1.5 mg/kg (intramuscular), the NAC was used in a dose of 150 mg/kg (intraperitoneal) and the antivenom serum in the ratio 1:3 (v/w) (intraperitoneal). Testicular gene expression was verified by RT-qPCR for the genes of antioxidant enzymes: glutone synthetase (Gss), catalase (Cat) and superoxide dismutase 2 (Sod2). V group had lower epididymal weight than groups V+S and V+NAC (P=0.0079). There was no significant difference in the relative gene expression of Gss and Sod2 in the testicles among the experimental groups. V+S, NAC and V+NAC groups showed higher relative gene expression of the Cat gene when compared to the V group (P=0.0114). It is concluded that NAC increases the gene expression of Cat in testicles of rats, as well as the antivenom, presenting an antioxidant potential for snakebite treatment.
Instituição: Universidade do Oeste Paulista
Tipo do documento: Dissertação2023-09-22T00:00:00ZExpressão de mediadores inflamatórios em testículos e dosagem hormonal de ratos espontaneamente hipertensos submetidos ao treinamento intervalado de alta intensidadeNespolo, Ronivania Jenuario Silvahttp://bdtd.unoeste.br:8080/jspui/handle/jspui/15572024-03-15T04:02:21Z2023-11-22T00:00:00ZTítulo: Expressão de mediadores inflamatórios em testículos e dosagem hormonal de ratos espontaneamente hipertensos submetidos ao treinamento intervalado de alta intensidade
Autor: Nespolo, Ronivania Jenuario Silva
Primeiro orientador: Giometti, Ines Cristina
Abstract: Hypertension is an age-related non-communicable chronic disease that causes a decline in the functioning of target organs, including the testis, as it is associated with increased oxidative stress and inflammation. Inflammatory cytokines increase with hypertension and cause inflammation and tissue injury. High-intensity interval training (HIIT) has shown promise as a non-pharmacological treatment for hypertensive individuals, but it is still unknown how it would act on the testicles of hypertensive individuals. The aim of this study was to investigate the effect of HIIT on testicular inflammation and on plasma hormone concentration in spontaneously hypertensive rats (SHR). Male SHR were divided into two groups: SHR (control, n=9); and HIIT (SHR undergoing HIIT on a treadmill, 5 days/week, for 8 weeks, n=10). Systolic blood pressure (SBP) was checked in the animals. At the end of the experiment, the animals were euthanized and the plasma and testes were collected. Fragments of one of the testicles of the rats were frozen and then used for RNA extraction using the TRIzol® protocol. Gene expressions of inflammatory mediators (Tnfα and Il6) were quantified by RT-qPCR. The combination of two reference genes (Hprt1 and Rps18) was used to normalize the reaction. The other testicle of the rats was used to make slides for the immunohistochemistry of TNFα and IL6. Plasma dosages of testosterone and corticosterone were performed by radioimmunoassay and prolactin by enzymeimmunoassay. The statistical analysis performed was unpaired Student's t test, except for testosterone which was the Mann-Whitney test (P<0.05). Animals that participated in HIIT had a lower SBP value. The groups did not show significant difference in the concentration of plasma hormones. The HIIT group had lower TNFα and IL6 immunostaining than the SHR and lower systolic blood pressure (P<0.05). It is concluded that HIIT for two months is effective in reducing inflammatory mediators in the testes of hypertensive rats.
Instituição: Universidade do Oeste Paulista
Tipo do documento: Dissertação2023-11-22T00:00:00ZImpacto da exposição perinatal de desreguladores endócrinos ambientalmente relevantes sobre a foliculogênese e remodelamento ovariano de ratas adultasOliveira, Gabriel Rodrigues Leal dehttp://bdtd.unoeste.br:8080/jspui/handle/jspui/15562024-03-15T04:02:20Z2023-10-06T00:00:00ZTítulo: Impacto da exposição perinatal de desreguladores endócrinos ambientalmente relevantes sobre a foliculogênese e remodelamento ovariano de ratas adultas
Autor: Oliveira, Gabriel Rodrigues Leal de
Primeiro orientador: Castilho, Anthony César de Souza
Abstract: Endocrine disruptors (EDs) are chemical substances ubiquitous in the human and animal environment that can disrupt the endocrine system and affect reproductive processes such as ovarian folliculogenesis. In this study, we investigated the effects of a mixture of endocrine disruptors (ED -mix) based on human environmental exposure on ovarian folliculogenesis and ovarian tissue organization in adult rats whose mothers were exposed to this mixture during gestation and lactation. To achieve this objective, pregnant females of the Sprague-Dawley line were randomly divided into two experimental groups: (i) the control group (Control), which received an oral administration of 2ml/kg corn oil, and (ii) the ED mix group, which received an oral administration of 32.11mg/kg/day of a mixture of twelve compounds (phthalates, pesticides, UV filters, bisphenol A, butylparaben) diluted in 2ml/kg corn oil Pregnant and lactating rats were treated from gestational day 7 (GD7) to postnatal day 21 (DPN21). After weaning, on PND22, there was a pause in the induction of mixtures of these compounds, and the females of the F1 offspring were sacrificed on postnatal day 360. The ovaries were removed, dissected, and examined histologically. Blood samples were also taken from the animals to determine hormone levels of estradiol and progesterone. Hematoxylin-eosin and picrosirius-stained tissue sections were examined for morphologic characteristics, measurement of fractal dimension, and quantification of total collagen, including type I and III fractions. The effects of exposure to the ED mixture during the fetal and lactation periods were assessed by Student's T test, with differences considered significant at P≤ 0.05. The results indicate that the mixture of endocrine disruptors led to an increase in the proportion of type I collagen fibers and III, a decrease in the number of follicles and corpora lutea, and a decrease in serum levels of 17β-estradiol and progesterone in F1-generation females. Overall, these results highlight that multigenerational exposure contributes to ovarian fibrosis in F1 generation rats and impairs antral follicle development, ovulatory capacity, and corpus luteum formation.
Instituição: Universidade do Oeste Paulista
Tipo do documento: Dissertação2023-10-06T00:00:00Z