http://bdtd.unoeste.br:8080/jspui/handle/tede/1 Pró Reitoria de Pesquisa e Pós-Graduação 2026-03-27T20:09:13Z http://bdtd.unoeste.br:8080/jspui/handle/jspui/1758 Título: Efeitos no tecido ósseo de ratos submetidos à exposição de desreguladores endócrinos durante a vida fetal e a lactação Autor: Thomaz, Gustavo Estevam Nóbrega Primeiro orientador: Nakagaki, Wilson Romero Abstract: Substances called endocrine disruptors (EDs) can compromise bone homeostasis. They mimic or block the action of natural hormones, interfering with aspects of their synthesis, secretion, and associated processes, such as the regulation of cell development for the formation of the body's tissues. The aim was to evaluate the bones of 420-day-old rats, offspring of rats fed a mixture of these compounds, from the 7th day of gestation to the 21st day of lactation, with consequent weaning of the offspring and the end of exposure to these foods. The animals were divided into two groups: the Control Group and the Endocrine Disruptors (EDs) Group. The pregnant or lactating rats received the treatment on the 7th day of gestation until the 21st postnatal day. The male pups were weaned on the 22nd day. They were then given only water and food until they were euthanized at 14 months. The femurs were then evaluated by mechanical and Raman analysis (spectral band ratios of 430/1272, 960/1660 and 1070/1660). The maximum resistance force and absorbed energy were similar between the two groups. However, the Endocrine Disruptors group proved to be 16.4% more deformable and 22.1% less rigid compared to the Control group. For Raman spectroscopy, the three band ratios analyzed were different between the groups. These bands were higher in the EDs group, indicating alterations in the bone matrix. Exposure to EDs during pregnancy and lactation led to impaired bone quality in adulthood. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação 2025-05-15T00:00:00Z http://bdtd.unoeste.br:8080/jspui/handle/jspui/1757 Título: Verificação da eficácia de um programa para o desenvolvimento da autoeficácia e autorregulação em estudantes de medicina Autor: Costa Filho, José de Oliveira Primeiro orientador: Murgo, Camélia Santina Abstract: The study is linked to the research line: "Training and Action of the Teaching Professional and Educational Practices" and to the research group "Construction of Subjectivity Processes in the School Context," of the Graduate Program in Education at Unoeste. Self-efficacy (SE) and Self-regulation (SR) play an important role in the implementation of effective learning. In this sense, the study sought to construct and verify the effectiveness of a program for the development of SE and SR in medical students. To this end, four studies were developed. In the first, a systematic literature review was conducted to identify the factors that impact SE in medical education, using the following databases: Virtual Health Library (VHL), Public Medline (PubMed), Brazilian Digital Library of Theses and Dissertations (BDTD), and CAPES Portal, covering the period from 2015 to 2020, resulting in twenty selected studies. It was concluded that SE is essential for the development of effective learning, and is associated with good emotional state, the development of teaching/learning strategies, and performance. In the second study, aiming to identify which SR factors are involved in medical education, a systematic literature review was performed using the databases: VHL, PubMed, CAPES Portal, and ERIC, covering the period from 2013 to 2023, resulting in the inclusion of twenty-two articles. The results indicated an association between SR and SE, intrinsic motivation, mastery goals, the use of cognitive and metacognitive strategies, and the use of co-regulatory networks, concluding that the promotion of SR is relevant in medical education. The third article aimed to evaluate the feasibility of a program for the development of SE and SR, construct the program, and partially apply it as a pilot study. The feasibility was confirmed by the criteria: acceptability, demand, implementation, adaptation, integration, practicality, expansion, and limited tests of efficacy. The program was built with eight sessions for application over one academic semester. The pilot study was applied to two groups of 12 students, each developing two sessions. The results demonstrated ease of participation, satisfaction, and appropriate timing, concluding that the program was feasible, appropriate, and operational. The fourth study, an intervention research, evaluated the effectiveness of the program constructed in study 3, named “Programa Elevare.” Fifty students participated, divided into two groups (experimental and control). The Self-Efficacy in Higher Education Scale and the Self-Regulation of Learning Processes Inventory were used, applied at pre-test, post-test, and follow-up in both groups. The experimental group also completed the session evaluation forms and the program evaluation questionnaire. The psychometric analysis demonstrated that the program was effective with a significant impact on the experimental group, especially in the dimensions of Academic Self-Efficacy, Self-Efficacy in Social Interaction, Self-Efficacy in Proactive Actions, and Self-Regulation, with significant differences over time, maintained up to 6 months after the end of the intervention (follow-up). Ultimately, it was concluded that the program was effective in promoting the improvement of levels of SE and SR in medical students. Instituição: Universidade do Oeste Paulista Tipo do documento: Tese 2025-04-02T00:00:00Z http://bdtd.unoeste.br:8080/jspui/handle/jspui/1756 Título: Devires da diferença: pensar o cinema para a educação Autor: VOLTARELI, Jucimara Pagnozi Primeiro orientador: Santos, Danielle Aparecida do Nascimento dos Abstract: This thesis seeks to deepen the theoretical discussions initiated during the Master’s in Education completed in the same Graduate Program in Education at the Universidade do Oeste Paulista (Unoeste), which focused on the representation of disability in Brazilian documentaries through Gilles Deleuze’s ideas on cinema. At this stage, the focus shifts to the cinematographic experimentation of a child with Autism Spectrum Disorder (ASD). The aim is to develop a shared philosophy through the creation of a short film that reflects on the multiplicity of philosophical thought and the aesthetic experience of this child. The thesis adopts the structure of plateaus, in which the text mirrors creative processes developed over four years, initially fostering philosophical discussions and subsequently documenting the lived experimentation. With this thesis format, we aim to make the research accessible in multiple ways. The methodological approach is qualitative, combining bibliographic research and the production of an experimental short film, with emphasis on the concept of becoming and on the analysis of philosophical and cinematographic studies on aesthetics, particularly regarding the normalization of people with disabilities in cinema, grounded in the philosophy of Deleuze and Guattari. From these experiences, the thesis seeks to address educational issues not usually contemplated by traditional science, proposing an educational aesthetics that fosters inclusive education, deviating from conventional standards and enabling new ways of thinking about difference. We conclude that subversive signs and affects, made possible through cinema, can contribute to inclusive education, often neglected in traditionalist educational approaches. Instituição: Universidade do Oeste Paulista Tipo do documento: Tese 2025-09-23T00:00:00Z http://bdtd.unoeste.br:8080/jspui/handle/jspui/1755 Título: Identificação e caracterização molecular da família das expansinas no fruto do Coffea arábica L. Autor: Lobo, Luís Gustavo Gomes Primeiro orientador: Santos, Tiago Benedito dos Abstract: The mechanism of fruit development in coffee plants is characterized by intense cell division, elongation and relaxation of the cell wall, processes related to the action of certain proteins, including expansins. Expansins (EXPs) represent a gene family and symbol in four subfamilies: α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA) and expansin-like B (EXLB). The aim of this study was to characterize expansins using bioinformatics tools and the molecular characterization of coffee fruits in the perisperm, endosperm and pericarp phases. The characterization of these genes began with the collection of information contained in the NCBI (National Center for Biotechnology Information) and Phytozome databases. Then, the following tools were used: ExPASsy and GRAVY (for physicochemical information); PlantmPLoc (subcellular localization); TAIR (orthologous genes); Gene structure: GSDS (exon/intro); MapGene2chromosomoe (MG2C- physical mapping of chromosomes); sequence alignment using MEGA 11 (phylogenetic analysis); Phyre2 (3D protein structures); MEME (Multiple Em for Motif Elicitation- conserved domains); PlantCARE (gene regulatory regions); and TBtools information on Ka/Ks (non-coincident and symbolic) and collinearity test. For gene expression, C. arabica L. grains were found at four development stages (30 DAF, 90 DAF, 120 DAF and 180 DAF) and subjected to qRT- PCR (quantitative real-time reverse transcription PCR) analysis. Forty-five expansin genes were characterized in silico and their physicochemical patterns contained a variation of 240-300 amino acids (aa), molecular weight 21.18 to 32.27 kDa, isoelectric point (pI) 4.35 to 9.68, and all were located in the cell wall. However, it was observed that their appearances seemed hydrophobic. The gene structure (exon/introns) varied from 2/1 to 6/5. The phylogenetic relationship classified them according to the four expansin subfamilies, forming five groups. The genes were unevenly distributed in the 11 chromosomes of C. arabica L.. The conserved domains varied from two to five. The DPBB and CBM63 domains were described in their 3D structures of each protein. In the synthesis of the CaEXPA genes, it showed greater similarity with the genomes of Oryza sativa and Arabidopsis thaliana, and the duplication of some genes was positive in the Ka/Ks analysis. The qRT-PCR result showed that the CaEXPA1, CaEXPA2, CaEXPA4, CaEXPA6, CaEXLA10, and CaEXLB11 genes were expressed during the four periods of fruit development. The CaEXPA1 gene showed greater pressure during 30 DAF, 90 DAF, and 120 DAF, while the CaEXPA4 gene was the most expressed among the genes evaluated, having a high expression in all periods of coffee bean development. This information establishes the basis for future research on the functional characteristics and molecular mechanisms of expansion genes in C. arabica L. in different coffee bean development projects. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação 2025-04-16T00:00:00Z