TEDE Coleção: Mestrado em Ciência Animal

Influência do treinamento aeróbico e resistido na síndrome metabólica, desempenho físico, estresse oxidativo e componentes da matriz extracelular muscular: estudo pré clínico

Fri, 16 Oct 2026 00:00:00 GMT

Título: Influência do treinamento aeróbico e resistido na síndrome metabólica, desempenho físico, estresse oxidativo e componentes da matriz extracelular muscular: estudo pré clínico Autor: Ferreira, Isabelle Tiburcio Pecin Primeiro orientador: Pacagnelli, Francis Lopes Abstract: Introduction: Metabolic syndrome (MS), characterized by obesity, insulin resistance, hypertension, and dyslipidemia, can cause muscle changes, oxidative stress (OS), and extracellular matrix (ECM) disorganization, with functional impairments. Physical exercise is indicated as a treatment; however, the most effective modality remains to be defined. Objective: To investigate the impact of two exercise modalities on the components of MS and their influence on skeletal muscle, evaluating OS biomarkers and ECM components. Methods: Wistar rats (n = 40) were distributed into two groups: control diet (C) or high-fat diet (MS) for 20 weeks. After characterization of MS, the animals were redistributed into four groups (n = 10): control (C), sedentary MS (MS), MS + resistance exercise (MS + RE) and MS + aerobic exercise (MS + AE), with an 8-week intervention, 5 days a week. RE: 50/75/90/100%, 4 climbs. AE: 80%8min+20%2min, 30–60 min. Food intake, weight gain, biochemical profile, functional capacity, OS markers: carbonylation, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and gene expression of ECM components (collagens and metalloproteinases) in the soleus and EDL muscles were evaluated. Statistical analysis used parametric tests: Student's t, ANOVA/Tukey, MANOVA/Bonferroni or nonparametric: Kruskal-Wallis/Dunn, (p<0.05). Results: MS was confirmed because it presented higher weight, blood glucose, blood pressure and triglycerides (p<0.05). MS reduced functional capacity and both exercise modalities improved physical performance between the pre- and post-training moments. The groups undergoing both types of training reduced weight, blood glucose, and blood pressure, while only aerobic exercise reduced % adiposity and triglycerides. The protocols reduced MDA and showed no change in carbonylation for both muscles evaluated. SOD levels increased in both exercise modalities in the soleus muscle, with no change in the EDL, and both muscles increased CAT only during resistance exercise. There were no changes in muscle gene expression. Conclusion: Both exercise modalities attenuated the effects of MS on muscles; aerobic exercise promoted greater benefits in functional capacity, and resistance exercise in redox balance for both muscles. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Comportamento das variáveis da dimensão fractal em tecido cutâneo cicatricial submetidos a diferentes métodos de indução de ferida e tratamentos: revisão sistemática de escopo

Tue, 27 May 2025 00:00:00 GMT

Título: Comportamento das variáveis da dimensão fractal em tecido cutâneo cicatricial submetidos a diferentes métodos de indução de ferida e tratamentos: revisão sistemática de escopo Autor: Vasconcelos, Luana Bezerra de Medeiros Primeiro orientador: Santarém, Cecília Laposy Abstract: Fractal dimension has proven to be a tool capable of interpreting diagnostic areas of biological tissue in order to understand therapeutic responses and their mechanisms in the process of wound induction and healing. However, understanding the behavior of fractal dimension variables is still inconclusive regarding the treatment of skin lesions, since different types of intervention seem to influence different patterns of inflammatory aspects and cell permeability. The present study aimed to systematically search databases and select preclinical studies regarding fractal analysis in the healing process of skin wounds. The search strategy included the Medline databases via OVID, EMBASE, Web of Science and CINAHL, considering the following terms: healing, wounds, collagen, rats, rabbits, burn, morphometry, histology and immunohistochemistry. The study selection process consisted of selecting the title, then the abstract and, finally, reading the full text. To control and standardize the study selection processes, the Rayyan platform and an Excel form were used so that the process would be blinded and standardized among the authors for subsequent double checking. A total of 407 articles were extracted from the databases. Of these, 69 were duplicates and were therefore excluded. After reading the titles and abstracts, 8 studies were included for full reading. Seven preclinical studies were included in this review because they met all the inclusion criteria, such as fractal analysis performed on tissues of rats, mice or rabbits, through induction of burn or dermoscopic cut wounds or emission of X-rays. Among the 7 studies found, the behavior of the fractal analysis was shown to be variable and dependent on the wound induction process, with the burn procedure being the one that presented the most differentiated results, due to the loss of tissue fractality related to thermal denaturation. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Associação do biocurativo e fibrina rica em plaquetas favorece a colagenização de feridas experimentais em coelhos

Tue, 13 May 2025 00:00:00 GMT

Título: Associação do biocurativo e fibrina rica em plaquetas favorece a colagenização de feridas experimentais em coelhos Autor: Faria, Ana Rosa Vieira de Primeiro orientador: Santarém, Cecília Laposy Abstract: Dermal tissues, which are essential for the integrity of the skin, are mainly composed of collagen, a vital protein that plays a crucial role in the wound healing process. As the skin regenerates, the quantity, organization and type of collagen change, directly influencing the strength of the healed skin. In the search for more efficient solutions for the healing of serious wounds, biodressings made from natural polysaccharides are gaining prominence as promising alternatives. Among these materials, chitosan, a polysaccharide obtained from chitin, stands out for its structural similarity to components of dermal tissue. Its ability to accelerate healing is remarkable, as it stimulates the expression of genes linked to the formation of blood vessels and the deposition of collagen. In addition, chitosan acts broadly in the healing process: it promotes wound coagulation, minimizes inflammation and encourages the proliferation of fibroblasts, cells essential for tissue regeneration. The aim of this study was to characterize the efficacy of the combination of biodressings with autologous platelet-rich fibrin in the regeneration of dermal wounds during 21 days of treatment, using fractal analysis of collagen as a quantitative measure of the structure. Skin biopsies from 18 rabbits were stained by the Picrosirius method and subjected to microscopic reading. The fractal dimension was estimated by the box-counting method using ImageJ software. A significant organization in collagen production was observed, evidenced by a lower density of collagen fibers and a regular morphological pattern. It is concluded that the combination of the membrane with FRPa can be an effective strategy to optimize wound healing, standing out as a promising alternative in the context of tissue regeneration. Future studies can explore different formulations and application times to maximize the efficacy of this treatment. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Influência positiva da combinação de biocurativo e fibrina rica em plaquetas autóloga na cicatrização de feridas dérmicas induzidas em coelhos

Mon, 24 Feb 2025 00:00:00 GMT

Título: Influência positiva da combinação de biocurativo e fibrina rica em plaquetas autóloga na cicatrização de feridas dérmicas induzidas em coelhos Autor: Moreira, Mônica Rafaela da Gama Primeiro orientador: Santarém, Cecília Laposy Abstract: Technological advances have led to the development of a wide variety of dressings intended for different types of wounds, aiming to address the four phases of healing. The ideal dressing should promote rapid healing, preserve skin hydration, be oxygen-permeable, non-adherent, hypoallergenic, and provide an effective barrier against contaminants, all at an affordable cost and with minimal discomfort to the patient. Biopolymers, such as chitosan, are widely used in wound treatment due to their biocompatibility, biodegradability, and similarity to macromolecules recognized by the body. However, many biopolymer-based formulations still face challenges. Thus, the combination of these materials with biomaterials such as Platelet-Rich Fibrin (PRF) is seen as a promising strategy for the future of wound healing. The present study aimed to characterize the histomorphometric effects of using biomaterials, with or without the bioactive dressing of chitosan complexed with xanthan and β-glucan, on the healing of experimentally induced wounds in rabbits. To that end, skin biopsies from 24 rabbits were evaluated on days 7, 14, 21, and 28 of the experiment. The results showed that aPRF significantly reduced the incidence of necrosis. On the other hand, when this biomaterial was associated with the dressing, a lower incidence of hemorrhage and a greater amount of inflammatory infiltrate were observed throughout the experimental period. It can be concluded that both the biodressing and PRF exhibit their best effects when used separately and at different times. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação