TEDE Coleção: Mestrado em Ciência Animal

Prevalência de sequelas pós-Covid-19 no aparelho locomotor de profissionais de saúde: revisão sistemática da literatura

Thu, 28 Aug 2025 00:00:00 GMT

Título: Prevalência de sequelas pós-Covid-19 no aparelho locomotor de profissionais de saúde: revisão sistemática da literatura Autor: Pontes, Marco Antonio Souza Primeiro orientador: Nakagaki, Wilson Romero Abstract: Introduction: Healthcare professionals were highly exposed to COVID-19 infection and vaccine administration during the pandemic. In addition to known systemic effects, musculoskeletal complaints such as myalgia, fatigue, joint pain, and stiffness have been reported, possibly associated with both infection and post-vaccination responses. These conditions may compromise functional capacity and quality of life in this population. Objective: To investigate the prevalence, affected tissues, most common body regions, and motor dysfunctions associated with musculoskeletal sequelae in healthcare professionals with COVID-19 or adverse effects following vaccination. Methods: A systematic review was conducted in PubMed, Embase, and Web of Science databases. Cross-sectional studies evaluating healthcare professionals with confirmed COVID-19 and musculoskeletal sequelae were included. Two independent reviewers performed study selection using Rayyan software. Data extraction was conducted using a standardized form, and methodological quality was assessed with the adapted Newcastle-Ottawa Scale. Due to methodological heterogeneity, a meta-analysis was not feasible; results were summarized narratively. Results: Five studies published between 2021 and 2023 were included, comprising 2,979 participants, mostly women (n = 1,963). Studies were conducted in countries including the Netherlands, United States, Ethiopia, Sweden, and Nepal. The most reported symptoms were myalgia, joint stiffness, spasms, and localized pain, mainly in the neck, back, and shoulders. Pain intensity ranged from mild to moderate (3–4 on the visual analog scale). Three studies assessed musculoskeletal adverse effects following vaccination, identifying local pain, functional limitation, and cases of subacromial bursitis and biceps tendinitis confirmed by ultrasound. Symptoms lasted from 4 hours to 7 days. The included studies were classified as having moderate methodological quality. Conclusion: Healthcare professionals may experience persistent musculoskeletal symptoms following COVID-19 infection or vaccination, with varying functional impact. Despite methodological limitations and lack of data on long-term disability, the findings highlight the importance of clinical and functional monitoring of healthcare workers in the post-pandemic period. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Influência da variabilidade geográfica sobre o potencial neurotóxico e reatividade cruzada com antiveneno comercial de venenos de subespécies de Crotalus durissus (Viperidae: Crotalinae) distribuídas no Brasil

Mon, 23 Feb 2026 00:00:00 GMT

Título: Influência da variabilidade geográfica sobre o potencial neurotóxico e reatividade cruzada com antiveneno comercial de venenos de subespécies de Crotalus durissus (Viperidae: Crotalinae) distribuídas no Brasil Autor: Demico, Poliana de Jesus Primeiro orientador: Floriano, Rafael Stuani Abstract: Envenomation by Crotalus snakes are known for causing significant neurotoxic and myotoxic effects, primarily attributed to crotoxin, a phospholipase A2 (PLA2) toxin that constitutes 70–90% of these venoms. Recent studies have demonstrated that geographic variability affects the PLA2 compositional profile of C. d. terrificus and C. d. collilineatus venoms, revealing a greater complexity of isoforms in specimens from the central-west and southeastern regions of Brazil. In this study, we assessed how geographic variability affects the neuromuscular activity of venoms from Crotalus durissus cascavella (CDV), C. d. collilineatus (CDC), C. d. ruruima (CDR), and C. d. terrificus (CDT), including the cross-reactivity of these venoms with commercial anti-Crotalus antivenom (AV) and PLA2 inhibition by varespladib (VPL). PLA2 activity was measured through spectrophotometry using 3-octanoyloxy benzoic acid as an artificial substrate. The mouse phrenic nerve-diaphragm preparation was set up in an Ugo Basile SRL myographic system under a tension of 1 g. Twitches were induced through supramaximal stimuli (0.1 Hz, 0.2 ms, ~6 V) and recorded with a DY1 force displacement transducer connected to a DataCapsule-Evo and LabScribe4 software. Results are expressed as mean ± SD (n=3–4). The CDR venom exhibited the highest PLA2 activity (75.5±2 ΔA425nm/min, n=3), while the CDV venom demonstrated greater sensitivity to VPL (0.3 mM), resulting in a ~97% inhibition of its PLA2 activity (p<0.05 compared to venom alone, n=3). CDC venom (10–100 μg/ml) induced significant neuromuscular facilitation, followed by a complete blockade within a 120 min incubation period. In contrast, CDV venom (10–100 μg/ml) exhibited greater potency at the neuromuscular junction, fully blocking twitch responses at all concentrations within 80–110 min. CDR venom resulted in a complete blockade only at the highest concentration of 100 μg/ml, which occurred within approximately 110 minutes. AV (antivenom:venom ratio 1:1.5 v/w) demonstrated partial efficacy against the venoms of both CDT and CDV (30 g/ml), avoiding the occurrence of 90% blockade for each. However, it exhibited limited cross-reactivity when tested against CDC and CDR (30 g/ml) venoms. In contrast, VPL at a concentration of 0.3 mM effectively inhibited the 90% blockade induced by both CDT and CDV (30 µg/ml) venoms, as well as avoiding the 50% blockade for CDC (30 µg/ml). The immunoreactivity with antivenom revealed the major enzymatic groups in these venoms, with C. d. cascavella and C. d. ruruima venoms being positive for crotamina. In conclusion, the venoms of these subspecies exhibit significant variability in their neuromuscular effects observed. Inhibition of PLA2 activity by VPL resulted in a marked reduction of the neuromuscular blockade induced by these venoms. Furthermore, VPL demonstrated greater efficacy than AV in mitigating the neuromuscular actions associated with CDC, CDT, and CDV venoms. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Avaliação microbiológica e molecular de Escherichia coli isoladas em carne moída bovina no interior de São Paulo

Fri, 10 Oct 2025 00:00:00 GMT

Título: Avaliação microbiológica e molecular de Escherichia coli isoladas em carne moída bovina no interior de São Paulo Autor: Alves, Maria do Socorro Primeiro orientador: Eller, Lizziane Kretli Winkelströter Abstract: Escherichia coli (E. coli) is a Gram-negative bacterium of the Enterobacteriaceae family with high genetic plasticity. Pathogenic strains produce toxins, adhesins, capsules, biofilms, and β-lactamases, which confer antimicrobial resistance. This study aimed to assess the presence of E. coli in ground beef sold in Presidente Prudente, São Paulo, and to characterize the virulence and resistance genes at the molecular level. A total of 51 samples of ground beef from butcher shops registered with the Health Surveillance Agency were analyzed. Bacterial identification was performed using microbiological, biochemical, and polymerase chain reaction methods. These methods included the detection of the papC, fimH, kpsMTII, fliC, blaTEM, blaSHV, blaCTX-M, and blaOXA genes, as well as phylogenetic classification (groups A, B1, B2, and D). Fifty-five isolates confirmed as E. coli were obtained, predominantly from group B2 (54.5%). The fimH (100%), papC (83.6%), and fliC (80%) genes were the most prevalent. All isolates showed biofilm formation with a weak adherence pattern. Resistance was low, with tetracycline resistance being the most prevalent (12.7%). Most isolates presented multiple resistance genes (mean of 2.27). It is concluded that ground meat can carry virulent and resistant strains of E. coli, highlighting the importance of hygiene measures and continuous microbiological surveillance. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Qualidade microbiológica, composição nutricional e estabilidade oxidativa de filés de tilápia congelados disponíveis no varejo

Tue, 24 Mar 2026 00:00:00 GMT

Título: Qualidade microbiológica, composição nutricional e estabilidade oxidativa de filés de tilápia congelados disponíveis no varejo Autor: Leite, Quézia Hadassa Machado Primeiro orientador: Zundt, Marilice Abstract: Global tilapia consumption reached approximately 7.1 million tons in 2024, with an annual growth rate of 5.7%, consolidating the species as one of the main global protein sources. However, the precariousness in food safety represents a massive economic barrier, with annual health costs exceeding US$ 14.1 billion and losses from recall events exceeding US$ 100 million for the industry. Given this scenario, the present study evaluated the physicochemical, microbiological, and oxidative quality of frozen tilapia fillets commercialized in retail markets over storage time (D0, D3, D7, and D14).The experimental design was completely randomized in a factorial scheme (brand × storage time), with data subjected to analysis of variance (ANOVA) and means compared using Tukey’s test (p < 0.05). A significant brand effect was observed on proximate composition, with variations in crude protein and extract contents, while moisture and ash showed no significant variations among brands and storage times. Although the glazing percentage predominantly met current legislation, microbiological analyses revealed critical non-conformities in part of the samples, regardless of storage time, indicating serious deficiencies in hygienic-sanitary control at the retail level. Oxidative stability, assessed by TBARS, showed progressive deterioration, with a significant increase in values at D7 and D14 and a significant brand × time interaction (p < 0.05). More than simple qualitative heterogeneity, the presence of pathogens and the observed oxidative instability expose vulnerabilities that directly compromise public health and the economic sustainability of the sector. The results reinforce that compliance with microbiological standards is not merely a legal requirement but a vital commercial survival strategy, demanding the immediate standardization of best practice protocols and the intensification of sanitary surveillance in the retail cold chain. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação