Efeito da aplicação de GH sobre a proliferação celular em testículos de ratos Wistar submetidos ao hormônio do crescimento sob treinamento resistido

Abstract

Growth hormone (GH) is often used indiscriminately by individuals looking for better physical performance, in order to increase muscle mass, which can lead to unwanted effects on the body. The aim of the present study was to investigate cell proliferation and gene expression linked to cell multiplication in testicles of Wistar rats that were subjected to growth hormone under resistance training. 32 male Wistar rats, aged 9 weeks, were used, divided into 4 experimental groups: CG (control group, n = 8); GHG (group of animals administered 0.2 IU / kg of GH, 3 times a week for 30 days, n = 8); TG (group of animals submitted to resistance training, 4 series of 10 jumps in water with 50% of the animal's weight, 3 times a week for 30 days, n = 8) and GHTG (group of animals submitted to resistance training and administration GH, n = 8). After the mice were euthanized, the testicles were collected to check for cell proliferation by the AgNOR technique and for the RT-qPCR of the target genes Ghr, Igf1 and Igf2. The statistical analysis of the data was ANOVA followed by Tukey, a statistically significant difference was considered with p <0.05. GHG rats showed greater weight gain than the other groups and greater testicular weight than GHTG (p <0.05). There was no significant difference in the count of nucleolus organizing regions and in the area of nucleus and nucleolus. There was also no significant difference in the expression of the target genes. The experiment is justified to find out if the use of GH in male rats would have cell multiplication and if the use of GH together with resistance training would genetically affect the male reproductive system. It was concluded that the application of GH under resistance training does not alter the testicular cell proliferation or the gene expression of Ghr, Igf1 and Igf2 in the testicles of Wistar rats in the analyzed period.