Análise cicatricial das lesões provocadas por queimaduras em coelhos e tratadas com fibrina rica em plaquetas autóloga

Abstract

Burns are a common type of wound, and its classification is based on the causal agent, exposure time and percentage of tissue affected. causing physical, emotional and financial damage to the burned patient. The use of dressings containing healing and anti-infective substances is one of the options for local therapy. Advances in the field of tissue engineering demonstrate that the use of autologous platelet-rich fibrin (aPRF) can have a great regenerative potential in complex wounds such as burns. The aim of the present study was to evaluate the healing response of wounds treated or not with aPRF after second-degree burns, sequentially and comparatively. Ten adult male New Zealand rabbits were used, divided into two groups: C-control group (n=5), which received standard treatment for burns (5g of 1% silver sulfadiazine cream), group T (n=5), treated with aPRF. To induce the lesions, burns were performed with a standardized iron bar (5 cm2), heated in a boiling water bath, for a period of 10 sec/quadrant. After this procedure, the animals received Tramadol hydrochloride (4.0mg/Kg, IM), twice a day for 3 consecutive days. Skin biopsies were performed at 7,14, 21 and 30 days post-lesion to assess fibrinoleukocyte crust, reepithelialization and percentage of contraction. Both groups at 7 days had 100% of the wounds with scabs, which evolved to absence at the end of 30 days of the experiment. The contraction of wounds treated with aPRF occurred 14 days after the burn, to the detriment of the control group (21 days), speeding up the healing process. When compared to the control group, it was found that the wounds treated with aPRF presented an onset of re-epithelialization at 7 days, unlike the control group, which started at 14 days. It is concluded that the use of aPRF provided a faster and more organized healing, being a viable biomaterial for the treatment of burns.