Avaliação das Fibras Colágenas de Feridas Dérmicas de Coelhos Tratadas com Diferentes Fontes de Plasma Rico em Plaquetas

Abstract

The platelet-rich plasma (PRP) is a promising resource in treatment of wounds, considered a non-toxic biomaterial, which when injected in the tissue, increases its regeneration capacity by stimulating growth factors and activating angiogenesis and thus to induce synthesis of collagen. Studies confirm the effectiveness of the autologous gel source of PRP, but the homologous and heterologous gel sources are alternatives when there are no conditions for obtaining the animal's own PRP, however, unknown whether the effect of using these sources in the organization of the collagen fibers during the process scar. Therefore, this study aimed to compare the effects on healing produced after performing dermal wound in rabbits treated with different sources of platelet-rich plasma (PRP) gel (autologous, homologous and heterologous). 36 biopsies of dermal wounds have been used, experimentally induced in 18 adult rabbits, clinically healthy, of New Zealand breed (n = 9 males, n = 9 females), treated and not treated with different sources of PRP. The wounds were sanitized and treated on 0,3,7,10,14 days, and, in 17º day, a biopsy of the wound has been made, for histologic evaluation and observation of the initial phase of remodeling. In order to determine the type of collagen, the biopsy slides passed through polarization technique with dye-red Picrosirius F3BA. Results confirmed that the source of homologous PRP gel proved to be as efficient as the source of autologous PRP gel if compared healing. Other important finding in this study was that heterologous PRP gel use provided a healing process as good as those provided by autologous and homologous gel groups. As tough PRP is rich in growth factors, even these being from other animals of the same species and also of different species, it is noteworthy that even when applied serially, the different types of PRPs have not caused negative effects, as rejection, for example. It has been concluded that the heterologous and homologous sources are safe alternatives and their use contributes positively to an adequate healing process.