Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Abstract

Snap Beans (Phaseolus vulgaris L) are highly valuable nutritionally, and it is cultivated by small, medium and big farmers. It represents a significant parcel of Brazilian economy concerning to the society. Culture emergency is a critical point in the production process and it is affected mainly by the water deficiency at this phase. The objective of this work was to simulate water deficiency in the germination beginning at the laboratory on seeds of snap beans ´Pérola´, using mannitol, CaCl2, MgCl2 and NaCl as osmotic in the potential of 0; -0.3; -0.6; -0.9 and -1.2MPa calculated with the aim of Van´t Hoff s equation and to evaluate the electrophorectical protein patterns of total soluble proteins by SDS-PAGE. Germination, vigour classification, roots and shoot dry weight and differential protein expression response was evaluated as parameters. The experimental design was completely randomized. Data was analysed by F test (ANOVA) and polynomial regression for the osmotic potential for each parameter evaluated. Banding pattern was evaluated by gel image. Simulation of deficiency, in laboratory, allowed the perception of the stress originated by NaCl in all parameter evaluated, validating the harsh of the NaCl and the lack of expression of low molecular weight proteins in this osmotic. 110 and 30kDa proteins were indicative of water stress, but not of salinity.