Multiplicação de Bacillus subtilis em vinhaça e viabilidade no controle de Meloidoginose em cana de açúcar

Abstract

This work aimed to evaluate the use of vinasse as a cultive medium for multiplication of Bacillus subtilis and the effect of your inoculation to control nematodes and growth of sugar cane grown in soil infested with the nematode galls (Meloidogyne spp.). Were developed in laboratory tests to define the best concentration of vinasse on the composition of culture medium to optimize the growth of B. subtilis. For the experiment with plants in the greenhouse soil used was collected in area of cultivation of sugarcane. Were established the following treatments: control, pure vinasse (50 m3 ha-1), Bacillus subtilis in aqueous suspension (50 m3 ha-1); B. subtilis multiplied in vinasse (50 m3 ha-1), multiplied in B. subtilis vinasse (100 m3 ha-1). The multiplication of Bacillus subtilis in the culture-based stillage was considered satisfactory when compared to the culture pattern. The application of B. subtilis without multiplication in vinasse promoted the growth of sugarcane and reduced reproduction of the nematodes in the root during the experiment. The use of B. subtilis, multiplied in vinasse not achieved the benefits found in the application of bacteria in aqueous suspension, with the stimulus to growth and control of nematodes in sugar cane.