Efeitos do gel de plasma rico em plaquetas heterólogo na cicatrização de feridas dérmicas padronizadas em coelhos

Abstract

The platelet rich plasma is a source of several growth factors and appears as a new biotechnology in tissue repair, with great therapeutic power. Patients with general health problems and the lack of volume of blood required for autologous production of concentrate, may be restricted to receive this innovative treatment. To overcome the limitation, this study evaluated the effects of heterologous platelet-rich plasma gel obtained from a healthy dog, in the healing of dermal wounds in rabbits by standard morphological, morphometric and histological analyzes. Two surgical incisions were made on the back of six rabbits using a punch of 8 mm diameter. Randomly one side was denominated as a Control (A), leaving the contralateral Treaty as (B). 0.9% saline solution and heterologous platelet-rich plasma gel were applied topically to the lesions (A) and (B) respectively, on days 0, 3, 7, 10 and 14 after surgery. Macroscopic observations recorded on the days mentioned above, including the 17th, which is also a biopsy for histological analysis was performed, demonstrated similarity between the lesions in relation to the study variables, pain sensitivity, color of wound edema, hyperemia, exudate, crust and granulation tissue. The process of contraction of the lesion area (A) and (B) similarly developed linearly, so that histologically complete reepithelialization was achieved in 90% of lesions (A) and 100% of the lesions (B), no differences statistically significant. Such differences were also not found between wounds in relation to other histological parameters analyzed: presence of crust, neovascularization, collagen, fibroblasts and macrophages, cell type and intensity of the inflammatory process. The results of this study suggest that the heterologous platelet-rich plasma gel was able to promote the healing of dermal wounds in rabbits, no harmful effects.