Efeito da administração de hormônio do crescimento associado ao treinamento físico nos parâmetros hematológicos de ratos Wistar

Abstract

In order to determine the hematologic changes resulting from the use of rhGH (recombinant human growth hormone), associated or not with physical training in rats, eighty Wistar rats, average weight of 211.8 g were randomly divided into four groups (n=20): CT (control group without physical training and without administration of rhGH), GH (group without physical training and with rhGH), TR (group with physical training and without rhGH) and TRGH (physical training group with rhGH). The animals in groups GH and TRGH received 0.2 IU/kg rhGH subcutaneously every two days during the 30 day period. Physical training was performed using a jumping protocol consisting of four sets of ten jumps with overload of 50% of body weight, three days a week, in a cylindrical container with 38 cm of heated water (30° C). At the end of 4 weeks, the animals were weighed, anesthetized and then killed by exsanguination for collection of blood for analyses. The following parameters were evaluated: number of erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, RDW (Red Cell Distribution Width), and leukocyte count. Red blood cell parameters did not change with exercise training and / or hormonal supplementation. In all groups RDW remained unchanged. The leukocyte count of the animals that have done physical training and were supplemented with hormone was higher when compared to sedentary rats (P < 0.05). The administration of rhGH at a dose of 0.2 IU/kg associated with exercise training increased the number of leukocytes, but did not change the remaining parameters of the hemogram in sedentary and active rats. It was not possible to detect an increase in erythropoiesis, through the use of rhGH, using these variables.