Efeito da concentração de bactérias probióticas como imunomoduladora da produção de anticorpos antirrábicos em bovinos vacinados

Abstract

This study assessed the effect of concentration of association a combination of probiotic micro-organisms added to the mineral mixture at different concentrations / day, immunomodulation production of serum antibodies against rabies virus in cattle vaccinated with a single dose of rabies vaccine. Forty-two male Nellore cattle, with 12 months aged, were randomly divided into three groups (n = 14): control group (CG) received 70 grams of mineral mixture / animal / day; 2 grams probiotics groups (G2P) and 8 grams (G8P) received 70 grams of mineral mix / animal / day added to grams of a combination of probiotic microorganisms, respectively 2 and 8 (Lactobacillus acidophilus, Streptococcus faecium, Bifidobacterium longum and Bifidobacterium thermophilum). Individual antibody titers were determined by the technique of neutralization in cell-based Rapid Fluorescent Focus Inhibition Test (RFFIT). We resorted to the analysis of variance on a route (one-way ANOVA) with contrasts using the Tukey method to determine if the experimental groups differed within each moment, and the paired t-test to determine whether differences occurred between times within each group and a 5% level of significance. There were statistically significant differences between the mean serum concentrations between the GC and G8P groups after 30 and 60 days after the first vaccination and 60 days, only the G8P kept 100% with titers of protective antibodies minimum. There was also improvement in the production of antibodies in the G2P group compared to GC after 30 and 60 days, but this difference was not statistically significant. The peak titers of antibodies in cattle for rabies in all groups were after the 30 days. The results obtained allow to attribute beneficial effect immunomodulation with the combination of probiotics in rabies humoral immune response and the concentration of the association of probiotic microorganisms in mineral mixture interfered beneficially in the humoral immune cattle primary response. There is evidence to assign the concentration of probiotic association to the effect on the maintenance of high levels of serum antibodies in cattle with a single dose of rabies vaccine.