Avaliação da genotoxidade do capsiate (Capsicum annum) e resveratrol suplementados em ratos wistar através do teste de micronúcleo

Abstract

This study aimed to evaluate the possible genotoxic effects of supplementation with capsiate extracted Pepper Capsicum annuum (sweet pepper), and the phenolic compound resveratrol extracted from grapes in Wistar rats by the micronucleus test in bone marrow. Sixty-four male rats, adult Wistar were divided into four groups (n = 16) and supplemented with: negative control group (GCN) - 0.5 ml of sodium chloride 0.9%; positive control group (GCP) - 50mg/kg cyclophosphamide; capsiate group (CG) - capsiate 10mg/kg diluted in 0.5 mL of 0.9% sodium chloride; resveratrol group (GR) - resveratrol 30mg/kg diluted in 0.5 mL of 0.9% sodium chloride. Only the PCM received intraperitoneal administration. The other groups were supplemented by gavage once a day for six weeks. At the end of six weeks the animals were anesthetized, sacrificed and the micronucleus test performed in bone marrow. The frequency of micronuclei in polychromatic erythrocytes of the BCM, PCM, GC and GR were 2.5, respectively, ± 0.53, 5.7 ± 1.03, 2.5 ± 0.73 and 2 ± 0.73 in a thousand cells. When comparing both groups there was statistically significant difference only GCP compared to other groups. It is concluded that supplementation at a dose of 10mg/kg capsiate and resveratrol at a dose of 30mg/Kg in daily frequency for 06 weeks did not produce genotoxicity in Wistar rats.