OCORRÊNCIA DE ESPÉCIES PATOGÊNICAS E APATOGÊNICAS DE LISTERIA EM SOLO DE PRAÇAS E PARQUES PÚBLICOS EM PRESIDENTE PRUDENTE, SÃO PAULO BRASIL

Abstract

To evaluate the presence of pathogenic and apathogenic strains of Listeria spp in urban parks and squares in the city of Presidente Prudente, 117 soil samples from 57 different georeferenced locations were evaluated for pH and inoculated in Fraser Broth for selective enrichment of Listeria spp. After incubation at 30° C for 7 to 21 days, broth with evidence of growth were plated on ALOA chromogenic agar. Bacterial colonies compatible with the Listeria were classified by morphotinctorial and biochemical criteria and evaluated for antimicrobial susceptibility by disk diffusion method. Listeria was isolated in 37 squares / parks (64.9%) and 57 of 117 samples (47.8%) of soil, of which 5 were classified as L. monocytogenes and 2 as L. ivanovii. There was a predominance of acidic pH in the city soil (mean = 5.44). The average pH did not differ between positive and negative isolates samples with Listeria spp (p = 0.5523). Spatial modeling techniques revealed no link between points with positive isolates and variability of soil pH. The type of square / park (infant, sports, garden and rest) did not affect the percentage of isolation for Listeria spp. Sandy soils tended to have lower percentages of isolation than clay soils. The seven isolated pathogenic were resistant to four or more antibiotics, including ampicillin (3/7 = 42.9%) and sulfonamides (5/7 = 71.4%). Considering that no collection point received recent fertilization, we concluded that pathogenic and apathogenic Listeria species are dispersed in the soil of urban areas, even under unfavorable pH and the absence of organic fertilizer use.