TEDE Coleção: Programa de Pós Graduação em Odontologia

Efeito do pré-tratamento da dentina com dimetilsulfóxido e da temperatura de volatilização do adesivo na resistência de união de pinos de fibra

Wed, 29 Jun 2022 00:00:00 GMT

Título: Efeito do pré-tratamento da dentina com dimetilsulfóxido e da temperatura de volatilização do adesivo na resistência de união de pinos de fibra Autor: SATO, Grace Mitiko Rosati Hori Primeiro orientador: Catelan, Anderson Abstract: Dentin pretreatment using dimethyl sulfoxide (DMSO) can result in higher resin infiltration and warm air to volatilize the adhesive can increase solvent evaporation, improving adhesion to root dentin. The aim in this "ex vivo" study was to evaluate the effect of pretreatment of root canal dentin using DMSO and the volatilization temperature of solvents of a universal adhesive system on the bond strength of fiberglass posts fixed with a dual curing resin cement in single-rooted teeth. Forty bovine roots (n = 10) with a length of 15 mm were used, which the root canal was endodontically treated and prepared (10 mm) for fixation of a fiberglass post (Exacto, Angelus) with the luting system Single Bond Universal + RelyX Ultimate (3M ESPE). Initially, the dentin was pre-treated with DMSO or distilled water (control) for 60 s and excess moisture was removed. Then, the adhesive system was applied in the self-etching mode and its volatilization is carried out at 23ºC (room temperature) or 40ºC (warm air stream) for 10 s. Light curing of the materials was carried out for the time recommended by the manufacturer with a polywave LED at irradiance of 1,000 mW/cm2. After the cementation of the fiber posts, the specimens were subjected to thermal cycling (10,000 cycles - 5 and 55ºC). Then, the roots were sectioned to obtain 1 mm thick slices of the cervical, middle, and apical thirds. Bond strength of the thirds was measured by the push out test using a universal testing machine. Failure pattern was evaluated under a stereomicroscope. Data were submitted to three-way split-plot ANOVA and Tukey's test ( = 0.05). Adhesive volatilization at 40ºC promoted an increase on bond strength compared to a temperature of 23ºC. The cervical third had the highest bond strength, followed by the middle third, with the lowest bond strength observed in the apical third, with statistical difference between them. Thus, it can be concluded that the solvent volatilization of adhesive system using warm air stream promotes an increase on bond strength of fiberglass post. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Efeito do probiótico Bifidobacterium animalis subs. Lactis HN019 como coadjuvante no tratamento periodontal em pacientes diabéticos tipo II.

Mon, 29 Aug 2022 00:00:00 GMT

Título: Efeito do probiótico Bifidobacterium animalis subs. Lactis HN019 como coadjuvante no tratamento periodontal em pacientes diabéticos tipo II. Autor: MOREIRA, Talita Magro Juvencio Primeiro orientador: Andraus, Luciana Prado Maia Abstract: The objective of this study was to evaluate the action of the probiotic Bifidobacterium animalis subs. lactis HN019 as an adjunct in the treatment of PD in type II diabetic patients. The sample of 17 participants was divided into a test group (n=6), which received scaling and root planing therapy (SRP) and probiotic fermented milk, and the control group (n=11), which received SRP and placebo sterilized milk. The volunteers ingested the probiotic milk and the placebo once a day for 90 days. The following parameters were evaluated: bleeding on probing (BoP), probing depth (PD), gingival recession (GR), clinical attachment level (CAL), glycated hemoglobin (HbA1c), fasting glucose, total cholesterol, HDL, LDL, triglycerides, body mass index (BMI) and waist-to-hip ratio (WHR). Data were collected on days 0 and 90 and analyzed statistically, considering 5% significance. As for the anthropometric parameters, there was a difference between the groups only in terms of hip circumference, with a decrease in the TG and an increase in the CG. There was an increase in the glycemic level in the TG and a decrease in the CG. In the other biochemical parameters, the TG showed a greater reduction in all evaluated parameters, when compared to the CG, but without significant difference. The improvement in PD parameters (mainly in moderate pockets), CAL and RG were greater in the TG, but without statistical difference. The effectiveness of the probiotic as an adjunct in the non-surgical treatment of PD in type 2 diabetic patients cannot be verified in this study and does not support the hypothesis of improve to control glycemia and lipidemia. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Estudo das interações entre a terapia dietética e a microbiota intestinal na doença inflamatória intestinal: Uma revisão sistemática e meta-análise

Mon, 26 Sep 2022 00:00:00 GMT

Título: Estudo das interações entre a terapia dietética e a microbiota intestinal na doença inflamatória intestinal: Uma revisão sistemática e meta-análise Autor: AVANCE, Aline Damasceno Primeiro orientador: Keller, Rogéria Abstract: Inflammatory bowel diseases (IBD) has shown an increase in incidence worldwide. The main proven risk factor for both IBD is a positive family history in 10-25% of patients. Crohn's disease (CD) can affect individuals aged 15 to 40 years and 50 to 80 years, has a higher percentage in women, and has increased around 15 times in the last decades. Ulcerative colitis (UC), the disease can start at any age. The peak of incidence seems to occur from 20 to 40 years and many studies show a second peak of incidence in the elderly. The pathogenesis of IBD is that genetically susceptible individuals develop intolerance to the dysregulated gut microbiota (dysbiosis) and chronic inflammation develops as a result of poor food triggers. Thus, the diet plays an important role in the modulation of the intestinal microbiota and can be applied as a therapeutic tool to improve the course of the disease. To carry out a systematic review and meta-analysis on the main interactions between dietary therapy, gut microbiota, and inflammatory bowel disease, to elucidate the main clinical outcomes of the disease after nutritional treatment. The present study followed the international model of systematic review and meta-analysis, following the rules of PRISMA. The inclusion criteria were clinical studies, involving randomized controlled, prospective, and retrospective (observational/epidemiological) studies, published from 2010 to 2020 on the main clinical outcomes of the nutritional treatment of inflammatory bowel diseases. The quality of scientific evidence in the studies covered was classified as high, moderate, low, or very low, according to the GRADE rules and the risk of bias according to a Cochrane instrument. The present study found seventeen important prospective randomized controlled clinical studies in the last ten years that showed the important role of dietary modulation in the control of IBD. In this sense, they showed important reductions in persistent intestinal symptoms, improvement in intestinal microbiota, reduction in circulating markers of inflammation, and improvement in quality of life, with p<0.05 (95% CI). Most of the studies listed were homogeneous (98.95%) in their clinical responses, suggesting the broad impact of dietary interventions on inflammation and clinical outcomes. The important role of modulation of the diet in the control and even in the remission of IBD was evidenced. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação

Exposição ocupacional de frentistas ao benzeno e alteração mutagênica em células bucais - revisão sistemática

Thu, 31 Mar 2022 00:00:00 GMT

Título: Exposição ocupacional de frentistas ao benzeno e alteração mutagênica em células bucais - revisão sistemática Autor: MANGANARO, Nathalia Lopes Primeiro orientador: Prado, Rosana Leal do Abstract: Gas station attendants are directly exposed to the inhalation of by-products of gasoline evaporation, such as benzene, which is harmful to health. This systematic review aimed to investigate the relationship between occupational exposure to benzene and the presence of mutagenic alterations in oral cells in gas station attendants. PICO was defined as P= gas station attendants, I= exposure to benzene, C= population without chronic exposure to benzene, O= mutagenic alteration. The search strategy was established by combining the descriptors: DNA damage, micronucleus, genotoxicity, mutagenicity tests, benzene, station attendants, gasoline and gas station. The search was performed in the following databases: PubMed/MEDLINE, Scielo, Scopus, Cochrane Library, Lilacs, Web of Science and Embase. The inclusion criteria were based on studies that performed the oral cell micronucleus test (BMn) in gas station attendants and a control group without exposure to benzene, literature review articles, clinical cases and no descriptive data were considered exclusion criteria. For the analysis of possible biases in the studies, the Newcastle-Ottawa and Axis scales were used. A total of 13,663 studies were found, of which only sixteen were selected because they met the inclusion and exclusion criteria. The studies suggested the presence of mutagenic alterations with increased frequency of micronuclei (Mn), binucleated cells (BN), karyorrhexis (KX), karyolysis (KY), pyknosis (PK), nuclear button (NB) and condensed chromatin (CC) in gas station attendants with 3 months to 30 years of chronic exposure to benzene, with more expressive alterations in gas station attendants with >5 years of working time. The increase in mutagenic alterations in oral cells of gas station attendants may indicate the risk of genotoxicity resulting from occupational exposure. Instituição: Universidade do Oeste Paulista Tipo do documento: Dissertação