Suplementação com quercetina reduz a dislipidemia e a esteatose hepática em ratos alimentados com dieta obesogênica

Abstract

Obesity has been considered a worldwide epidemic by the World Health Organization that generates metabolic disorders, dyslipidemia, insulin resistance, hepatic steatosis and obesity is a risk factor for the development of chronic diseases such as Cardiovascular and diabetes mellitus type 2. The objective of this study was to evaluate whether quercetin has the potential to mitigate the deleterious effects of the obesogence diet such as dyslipidemia and hepatic steatosis, weight gain and deposition of adipose tissue in rats. We used 32 male Wistar rats fed with water and ad libitum ration and kept at 23 oC and light/dark cycle of 12/12 hours that were divided into 4 groups (8 rats/Group): GC fed with basal diet; GO Rats received obesogenic diet for 16 weeks and GOQ1 and GOQ2 rats received obesogence ration for 8 weeks without supplementation with quercetin, followed by another 8 weeks receiving obesogence ration of 10 and 20 mg of quercetin/day, respectively. The obesogence ration consisted of 50% of commercial balanced ration, 20% of fructose PA and swine lard to achieve a concentration of 30% ether extract. At the end the samples were harvested: blood for determination of serum concentrations of total cholesterol, HDL (High Density Lipoproteins), LDL (Low-Density Lipoprotein), AST and ALT (Alanino Aminotransferase) enzymes, liver for histopathologic analysis and Epididymal, mesenteric and retroabdominal fats for evaluation of the deposition of Fat in the rats. In the data analyses, ANOVA and Tukey or Kruskall-Wallis and Dunn were applied, with a significance of 5%. Rats from the GO group had dyslipidemia and hepatic steatosis. Rats from Groups GOQ1 and GOQ2 did not present dyslipidemia and hepatic steatosis, but only rats from the GOQ2 group presented reorganization of the hepatic trabecula. Supplementation with 20 mg of quercetin/day was the best dose capable of reducing dyslipidemia and hepatic steatosis in rats fed with obesogence diet.