Influência de irrigação subgengival com Camomila e Tansagem sobre o tratamento da doença periodontal experimental em ratos. Estudo microbiológico, histoquímico e imunoistoquímico

Abstract

Introduction: The present study evaluated the influence of subgingival irrigation with natural extracts of Chamomile and Tansage in addition to conventional therapy on the quantification of Colony Forming Units (CFU) and periodontal healing in experimental periodontitis (PE). Methods: Before PE induction, a biofilm sample was collected from the periodontal groove by means of a sterile paper cone on the lower left first molar of 72 rats. Subsequently, PE was induced in these teeth through the insertion of cotton thread in the subgingival region. After 7 days of PE induction, the bandage was removed. The animals were randomly divided into 3 groups: RAR - Root Scraping and Straightening and irrigation with 1 ml of saline; CAM - scaling and root planing and irrigation with 100 µg chamomile / ml of aqueous solution; and TAN - scaling and root planing and irrigation with 100 µg Tansagem / ml of aqueous solution. After the treatments, a new biofilm sample collection was attended to. Each group was subdivided into 3 subgroups (n = 8) and biofilms were collected from the periodontal sulcus of the teeth with induced PE and prior to euthanasia performed at 7, 15 and 30 days post-treatments. The CFU quantification was performed using biofilm samples. In addition, histomorphometric analyzes of the area of bone loss (PO) in the furcation region, percentage of mature and immature collagen fibers using Picrosirius red and osteoclastogenesis through tartrate-resistant acid phosphatase (TRAP), an activator receptor ligand nuclear factor kappa B (RANKL) and osteoprotegerin (OPG) by the indirect immunoperoxidase method. The data were statistically imposed (p <0.05). Results: CAM and TAN additional number of UFC less than RAR at 15 days. NHS due to secondary PO less than CAM at 7 days. CAM offering complete immature collagen fibers than the RAR and TAN groups at 15 days. CAM presented immunostaining for RANKL and Complete OPG greater than TAN at 15 and 7 days, respectively. Conclusion: Subgingival irrigation with phytotherapics supporting RAR reduces the amount of CFU and improves healing in the treatment of PE in rats.