Ação antimicrobiana do NeoMTA Plus em biofilmes simples e mistos de Enterococcus faecalis e Candida albicans : um estudo in vitro

Abstract

Objective: The aim of the present study was to evaluate the antimicrobial capacity of NeoMTA Plus on simple and mixed biofilms of Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans). Materials and Methods: 171 dentin blocks measuring 4x4x1mm were incubated in medium containing 1x107 cells/mL of C. albicans and/or 3,1x108 cells/mL of E. faecalis for 2 days. The blocks were randomly divided into 3 groups: group I - did not receive biomaterial; group II - received a sample of MTA after setting time, with the same width and thickness as the dentin block; and group III - similar to group II, but the biomaterial used was NeoMTA Plus. Biomaterials remained in contact with biofilms for 24 hours. in addition to the qualitative analysis of the structure of biofilms by scanning electron microscopy (SEM). After contact period, colony-forming units (UFCs) and metabolic activity (XTT) quantification analyzes were performed. Furthermore, qualitative analysis of the biofilm structure was performed through scanning electron microscopy (SEM). Data were analyzed and submitted to statistical analysis with a significance level of 5% (p <0.05). Results: For all groups it was confirmed the presence of metabolic activity (XTT) and UFCs, with no significant difference between groups (p> 0.05). Type of biofilm (simple or mixed) and biomaterial used (MTA or NeoMTA Plus) did not interfere the result. The biofilm structure remained homogeneous among the groups. Conclusion: Based on the present results, it can be concluded that NeoMTA Plus was not effective against simple or mixed biofilms of E. faecalis and C. albicans. Clinical relevance: New researches should be carried out – including the association of the aforementioned biomaterials with antiseptics and/or supporting therapies – to remove biofilms.