Perfil de sensibilidade microbiana de Staphylococcus psedintermedius isolado de cães com ceratoconjuntivite seca

Abstract

Keratoconjunctivitis sicca (KCS) or dry eye disease is an inflammatory condition that affects the ocular surface of dogs, reducing the aqueous component of the tear film. Possible complications include infections by endogenous bacterial agents that multiply due to changes in the ocular microenvironment. Staphylococcus pseudintermedius is a Gram-positive coconut producer of the plasma coagulase enzyme and is the main opportunistic agent involved in ocular infections secondary to CCS. The present study was divided into two articles. The first article evaluated 50 strains of Staphylococcus pseudintermedius isolated from 25 dogs with dry keratoconjunctivitis confirmed by Schirmer's tear test (TLS), tear film rupture test (TRFL) and green Lissamine (TLV) and fluorescein (TF) test. . Additionally, clinical and cytopathological scores of superficial eye smears were evaluated. The isolated strains were tested for sensitivity to calculate multiple antimicrobial resistance (IMR) index, expression of virulence factors (production of urease, gelatinase, dnase, hemolysin) and gene associated with biofilm production (icaA). Significant differences were found between secretion scores between animals with eyes colonized by urease-positive and uease-negative strains. Only 3 (6%) of the strains presented presence of the icaA gene. Twenty-four (48%) strains of S. pseudintermedius showed an IMR equal to or greater than 0.3 and a negative correlation between TLS and TRFL and IMR. The second article evaluated the sensitivity profile of 20 strains of Staphylococcus pseudintermedius isolated from eye secretion swabs of 60 dogs with dry keratoconjunctivitis confirmed by the Schirmer tear test (TLS) and Tear Film Break Test (TRFL). Sensitivity tests were based on the disc diffusion method. In addition, the D test was performed to evaluate the phenotype of clindamycin-induced resistance by erythromycin and the aggregate multiple resistance index (IMRA) was calculated. Low resistance ratios for Cephalexin (1/20 = 5%) and Imipenem (0/20 = 0%), intermediate for Clindamycin (5/20 = 25%), Chloramphenicol (6/20 = 30%), Erythromycin, were observed. (6/20 = 30%), Gentamycin (7/20 = 35%) and Vancomycin (3/20 = 15%) and high for Ofloxacin (10/20 = 50%, Penicillin G (10/20 = 50%) Tetracycline (12/20 = 60%) Seven of the 20 samples (35%) were positive on the D-test IMRA was 0.325 Four strains showed identical resistance pattern, characterized by sensitivity only to Imipenem, Cephalexin and Vancomycin Global resistance to antimicrobials revealed predominance of resistant strains in animal eyes This result is reinforced by the detection of three strains resistant to seven classes of antimicrobials, being sensitive only to Imipenem, Cephalexin and Vancomycin and may characterize risks of zoonotic transmission. of multiresistant agents from dogs to humans.The results indicate that eyes of dogs with CCS may be secondarily colonized by antimicrobial resistant strains commonly indicated for ophthalmic use, reinforcing the need to evaluate the in vitro sensitivity of S. pseudintermedius to support the therapeutic regimen.