Influência da ação dos probióticos associados com a fotobiomodulação no processo de reparo após o reimplante dentário

Abstract

The aim of the present study was to evaluate the association between photobiomodulation for surface treatment and the use of probiotics as a storage media in the repair process of dental tissues after late reimplantation. Fifty rats were divided into 5 groups and had their upper right central incisors extracted and treated, as follows: Negative Control Group, teeth immersed in saline solution for 30 min; Milk Group, teeth immersed in milk for 30 min; LLLT Group, teeth immersed in saline solution for 30 min and, after, teeth were photobiomodulated; Probiotic Group, teeth immersed in Bifidumbacterium HN019 for 30 min; Probiotic + LLLT Group, teeth immersed in probiotic for 30 min and, after, teeth were photobiomodulated. The teeth were reimplanted and after 60 days the animals were euthanized and inflammatory, reabsorptive and reparative parameters were histologically analyzed. Quantity and quality of collagen fibers were evaluated histochemically. Histological evaluation showed that the Probiotic + LLLT group had an inflammatory infiltrate with expressive intensity and extension and presence of root resorptions, results similar to those of the other groups (p>0.05). The amount of mature collagen fibers was greater than the immature ones for all groups (p<0.05) and there was no statistically significant difference between the evaluated groups (p>0.05). The association of photobiomodulation with probiotics can be an alternative treatment protocol for late tooth replantation, especially because the similar results to Milk group, traditionally recommended treatment. Within the limitations of the work and according to the parameters studied, it is concluded that the treatments used can be promising because they presented results similar to the Milk group. However, further studies should be carried out for its clinical indications.