Efeitos do tratamento precoce e contínuo de resveratrol associado à dexametasona em feridas cutâneas de ratos

Abstract

OBJECTIVE: To verify the effects of the association of the use of resveratrol and dexamethasone in prophylactic and continuous treatment in the healing process of rat skins. METHODS: Sixty male rats (Wistar) were randomly divided into four experimental groups (groups: n=15): Control (C), Dexamethasone (Dexa), Resveratrol (Resv) and Dexamethasone associated with Resveratrol (D+R). The lesion was performed after the 35th day of treatment with the aforementioned substances. The treatments were carried out continuously until the 3rd, 7th and 14th day-injury. For each step, the animals were taken and the wound was measured. After the maintenance of their animals, they were used for animals and domestic animals, the animals used as technical accessories, were used for animals and domestic animals. The quantification of mast cells (Toluidine Blue), Fibroblasts (Hematoxylin Eosin), Collagen type I and III (Picrosirius) and methanoloproteinases 2 and 9 by biochemical analysis. Statistical analysis used was two-way ANOVA with Bonferroni post-test and significance level p<0.05. RESULTS: The Dexa group had significant final weight loss in the periods of 3 days vs 7 days and 3 days vs 14 days. Wound contraction showed results for all groups. Mast cell quantification was significant only for the control group. For fibroblasts there were results for all groups that received treatment. Type III collagen project significant results for control group. The activities of ProMMP-2 showed values of -2 for the control group, MMP- for control and dexa, Pro-MMP-9 for the dexa and D+ groups as well as for MMP-9. The other results were not results. CONCLUSION: the preventive treatments of the lesion using the association of resveratrol and dexamethasone, the active resveratrol alone and separate groups were positive for the healing phases to the others. In addition, the active veratrol does not indicate natural effects or allergic effects to animals, which can be a promising substance in wound healing.