Determinação da ação antifúngica de madeira Pinus elliottii tratada com nanopartículas de prata

Abstract

Wood is a material used in civil construction in many ways and has a great aesthetic value. Problems that seriously impair its aesthetic and structural properties are the appearance of pests and the development of filamentous fungi, that is, molds, and other microorganisms. This work proposed to evaluate the antifungal action of silver nanoparticles (NPAg) against Aspergillus niger on Pinus elliottii wood. The synthesis of NPAg was carried out from the chemical reduction of silver nitrate (AgNO3) using sodium borohydride (NaBH4) and confirmed by the yellowish coloration and by UV-Vis spectrophotometry, with detection of the plasmonic peak at 400 nm. determination of the minimum inhibitory concentration (MIC) of NPAg on Aspergillus niger (CCCD-AA 001), then the antifungal activity of Pinus elliottii wood with NPAg was evaluated. The wood samples were cut into small 5x5x5mm cubes, sterilized and treated with different concentrations of AgNP, after the treatment they were placed in culture plates containing culture medium inoculated with Aspergillus niger (CCCD-AA 001) in order to obtain the formation of the biofilm, followed by plating to establish the CFU/mL and finally, the metabolic activity was verified through the reduction of 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) It was observed that the MIC was f53.56 ppm, all samples of wood treated with NPsAg inhibited the growth and metabolic activity of Aspergillus niger (CCCD-AA 001). It was possible to conclude that the wood samples impregnated with NPs prevent the proliferation of pests, fungi, bacteria and other microorganisms, which can bring benefits to the health of the inhabitants and the environment, providing quality and durability in buildings that use wood.