Efeito protetor da piperina no dano intestinal induzido por indometacina

Abstract

Non-steroidal anti-Inflammatory drugs (NSAIDs) are medications widely prescribed in clinical practice for the treatment of inflammation and pain, mainly in cases of chronic pain, osteoarthritis, rheumatoid arthritis, post-surgery and menstrual cramps. The mechanism of action of NSAIDs is based on the non-selective prevention of the enzyme Cyclooxygenase (COX) with protection from the conversion of arachidonic acid into Prostaglandins (PGs), which consist of a substance that mediates inflammatory reactions in the body. The classic use of NSAIDs is associated with adverse events and organ damage, especially gastric mucosa, small intestine, cardiovascular system, kidneys, liver, brain and respiratory tract. The objective of this work was to evaluate the protection of piperine in intestinal damage induced by Indomethacin. 18 male Mus musculus mice, 6-8 weeks old, were used. Intestinal damage was induced with indomethacin (10mg/ml) and co-treatment with piperine (20mg/ml), both orally. After 14 days, the animals were euthanized. Biochemical serological analysis was performed. Intestinal inflammation was evaluated based on macroscopic, histopathological and metagenomic analyses. Histopathological analysis demonstrated a reduction in inflammation of the small intestine (p<0.05) and disappearance of necrosis of the intestinal wall of the large intestine. The proportions of crypts and villi caused increased values in the group treated with piperine (p<0.05). An Aspartate aminotransferase (AST) value approximately six times higher was observed in the Indomethacin group (p<0.05). Regarding the intestinal microbiota, it was possible to observe an increase in gender diversity in the group treated with piperine (p<0.05). There was a 50% reduction in micronucleus formation with the administration of piperine 20mg/kg (p<0.05). It is concluded that co-treatment with piperine has great potential in remedying the side effects caused by NSAIDs.