Uso da Fibrina Rica em Plaquetas associada a curativo bioativo de quitosana/xantana/β-glucana na cicatrização de feridas experimentalmente induzidas em coelhos

Abstract

The use of Platelet-Rich Fibrin associated with a bioactive dressing of chitosan/xanthan/β-glucan in the healing of experimentally induced wounds in rabbits. Wound healing is a dynamic and rapidly growing field in the global market. Advances in technology have led to the development of a wide range of dressings for different types of wounds targeting the four phases of healing. The ideal dressing should promote rapid healing; preserve skin hydration; be oxygen-permeable, non-adherent, and hypoallergenic; and provide a barrier against contaminants - all at a reasonable cost and with minimal inconvenience to the patient. Therefore, the choice of the best dressing should be based on what the wound needs and what the dressing does to achieve complete regeneration and restoration of the skin structure and function. Biopolymers such as chitosan are widely used in wound treatment due to their biocompatibility, biodegradability, and similarity to recognized macromolecules. However, most biopolymer-based formulations still present several problems; thus, strategies to combine them with biomaterials like autologous Platelet-Rich Fibrin (PRF) represent the future of wound healing. Twenty-four rabbits were used to characterize the macroscopic changes in experimentally induced wounds treated with autologous platelet-rich fibrin with and without a bioactive dressing on morphological aspects associated with the healing process, on days 07, 14, 21, and 28. Thus, this study aimed to analyze the use of biomaterials with or without the bioactive dressing of chitosan complexed with xanthan and β-glucan in the healing of wounds induced in rabbits, characterizing the macroscopic and morphometric effects. It is concluded that aPRF proved to be more promising in relation to wound healing time, however, in morphological analyzes the use of the membrane did not provide a synergistic effect when associated with the biomaterial.