Associação do biocurativo e fibrina rica em plaquetas favorece a colagenização de feridas experimentais em coelhos

Abstract

Dermal tissues, which are essential for the integrity of the skin, are mainly composed of collagen, a vital protein that plays a crucial role in the wound healing process. As the skin regenerates, the quantity, organization and type of collagen change, directly influencing the strength of the healed skin. In the search for more efficient solutions for the healing of serious wounds, biodressings made from natural polysaccharides are gaining prominence as promising alternatives. Among these materials, chitosan, a polysaccharide obtained from chitin, stands out for its structural similarity to components of dermal tissue. Its ability to accelerate healing is remarkable, as it stimulates the expression of genes linked to the formation of blood vessels and the deposition of collagen. In addition, chitosan acts broadly in the healing process: it promotes wound coagulation, minimizes inflammation and encourages the proliferation of fibroblasts, cells essential for tissue regeneration. The aim of this study was to characterize the efficacy of the combination of biodressings with autologous platelet-rich fibrin in the regeneration of dermal wounds during 21 days of treatment, using fractal analysis of collagen as a quantitative measure of the structure. Skin biopsies from 18 rabbits were stained by the Picrosirius method and subjected to microscopic reading. The fractal dimension was estimated by the box-counting method using ImageJ software. A significant organization in collagen production was observed, evidenced by a lower density of collagen fibers and a regular morphological pattern. It is concluded that the combination of the membrane with FRPa can be an effective strategy to optimize wound healing, standing out as a promising alternative in the context of tissue regeneration. Future studies can explore different formulations and application times to maximize the efficacy of this treatment.