Análise da interação pós-biótico com biossensor e efeitos de nanocomposto probiótico na inflamação intestinal

Abstract

This thesis is structured in two chapters and explores different therapeutic approaches for gut protection, focusing on the use of probiotic nanocomposites and the relationship between postbiotics and biosensors in gut inflammation. The first chapter is a literature review that discusses the interaction between postbiotics and biosensors, addressing how postbiotics, produced by probiotics, can be used in the detection of inflammatory biomarkers and in the promotion of therapeutic effects. In addition, the review highlights the potential of biosensors as diagnostic tools in monitoring gut inflammation and the importance of integrating advanced technologies for the development of systems that allow rapid diagnosis and continuous monitoring of gut health in patients with Inflammatory Bowel Disease (IBD). The second chapter investigates the effect of chitosan nanocomposites combined with Pediococcus acidilactici CE51 on protection against intestinal lesions caused by indomethacin in mice. The nanocomposite was produced by ionic gelling and characterized for zeta potential (-7.86 mV), PdI (0.513±0.05) and mean diameter (358±18.38 nm). The stability of the compound was tested in the oral, gastric and intestinal phases of digestion. The results showed that the nanocomposite combined with P. acidilactici CE51 promoted a significant reduction in intestinal and liver damage compared to the group exposed to indomethacin alone, in addition to improving biochemical parameters, such as the decrease in serum levels of aspartate aminotransferase (AST) and urea.