Estudo clínico e laboratorial da intoxicação experimental pelo veneno da serpente Crotalus durissus terrificus em ratos Wistar tratados com soro antiofídico e Mikania glomerata

Abstract

The accident crotalic presents the largest lethality index when compared with other ophidic accidents of medical interest. The fractions of the crotalic venom characterize three activities with known clinical importance: neurotoxic, hemolytic and myotoxic activities. The present study aims evaluate the clinical and laboratorial aspects of the experimental intoxication with venom of Crotalus durissus terrificus in mice Wistar treated with antiophidic serum and aqueous extract of Mikania glomerata. The animals were divided in three groups with eighteen animals each one. Group GC: control; Group GVS: received 10mg/kg of venom saw intramuscular and 1mg of antiophidic serum saw intraperitoneal for each 50mg of crotalic venom; Group GVSM: received the same protocol to the group GVS and the aqueous extract of Mikania glomerata 10% at the dose of 1mL orally each hour totaling five administrations. They were observed moderate edema, moderate sedation, decrease of the locomotion, temperature, breathing frequency and heart frequency, however there was not evidence of coagulation alteration. In the comparison among the groups GVS and GVSM for all of the appraised parameters observed that it was significant for the statistical analysis the larger edema in the member inoculated in the animals of the group GVS and the return of the faster sedation degree for the animals of the group GVSM. The breathing frequency didn't differ among these two groups, but it was smaller in the animals of the group same GVS staying inside of normal values for the species. The locomotion decreased, but it didn't differ between the two groups and the temperature and heart frequency didn't differ for the statistical analysis, but the decrease of their values below the normal values for the species, more intense in the animals of the group GVS, was clinically important. We also concluded the need of larger studies with the plant Mikania glomerata in the accidents crotalic in more sensitive species to the venom